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elite abc kit pk6100 sections  (Vector Laboratories)


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    Structured Review

    Vector Laboratories elite abc kit pk6100 sections
    Elite Abc Kit Pk6100 Sections, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12897 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pk6100/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pm41787744-481-25-24
    Average 99 stars, based on 12897 article reviews
    elite abc kit pk6100 sections - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Choline supplementation in early life improves and low levels of choline can impair outcomes in a mouse model of Alzheimer’s disease
    Article Snippet: Chemical compound, drug , H 2 O 2 ; hydrogen peroxide , Cat# 95321; Sigma-Aldrich , , 1% w/v. .. Chemical compound, drug , Avidin-biotin complex (ABC) , Cat# PK6100; Vector Laboratories , , 1:1000. .. Chemical compound, drug , 3,3-Diamino-benzidine (DAB) , Cat# D5905; Sigma-Aldrich , , 0.5 mg/mL.

    Article Title: Choline supplementation in early life improves and low levels of choline can impair outcomes in a mouse model of Alzheimer’s disease
    Article Snippet: .. resource Designation Source or reference Identifiers Additional information Chemical compound, drug Paraformaldehyde (PFA) Cat# 19210; Electron Microscopy Sciences 4% Chemical compound, drug Normal goat serum Cat# S- 1000; Vector Laboratories 5% Chemical compound, drug Normal horse serum Cat# S- 2000; Vector Laboratories 5% Chemical compound, drug TRIS hydrochloride Cat# T3253; Sigma- Aldrich 97 g/8 L Chemical compound, drug Tris base (TRIZMA base) Cat# T1503; Sigma- Aldrich 22 g/8 L Chemical compound, drug H2O2; hydrogen peroxide Cat# 95321; Sigma- Aldrich 1% w/v Chemical compound, drug Avidin- biotin complex (ABC) Cat# PK6100; Vector Laboratories 1:1000 Chemical compound, drug 3,3- Diamino- benzidine (DAB) Cat# D5905; Sigma- Aldrich 0.5 mg/mL Chemical compound, drug Ammonium chloride Cat# A514; Sigma- Aldrich 40 ug/mL Chemical compound, drug D(+)- glucose Cat# G5767; Sigma- Aldrich 25 mg/mL Chemical compound, drug Glucose oxidase Cat# G2133; Sigma- Aldrich 3 g/mL Chemical compound, drug Gelatin Cat# G9391; Sigma- Aldrich 1% Chemical compound, drug Xylene Cat# 534056; Sigma- Aldrich Undiluted Chemical compound, drug Permount Cat# 17986- 01; Electron Microscopy Sciences Undiluted Chemical compound, drug Glycerol #G7893; Sigma- Aldrich 30% Chemical compound, drug Ethylene glycol Cat# 324558; Sigma- Aldrich 30% Chemical compound, drug Sterile 0.9% sodium chloride solution; saline NDC# 50989- 885- 17; Vedco, Inc Chemical compound, drug Dental cement Cat# 4734FIB, Lang Dental Mfg. .. Co Chemical compound, drug Lactated Ringer’s solution NDC# 099355000476; Aspen Veterinary Resources Ltd 50 mL/kg Chemical compound, drug Isoflurane NDC# 07- 893- 1389; Patterson Veterinary Chemical compound, drug Urethane Cat# U2500; Sigma- Aldrich 2.5 g/kg Chemical compound, drug Buprenorphine Buprenex; NDC#12496- 075705; Reckitt Benckiser 0.2 mg/kg Strain, strain background (Mus musculus) Tg2576 mice, C57BL6/SJL background Jackson Labs Stock# 100012 Software, algorithm ImagePro Plus V7.0 Media Cybernetics Software, algorithm Sirenia acquisition Pinnacle Technology RRID:SCR_016183 Software, algorithm Sirenia Seizure Pinnacle Technology RRID:SCR_016184 Software, algorithm Neuroscore Data Science International Software, algorithm Prism GraphPad RRID:SCR_002798 Software, algorithm Photoshop Adobe RRID:SCR_014199 Software, algorithm ImageJ NIH RRID:SCR_003070 Version 1.44 Software, algorithm G*Power G*Power RRID:SCR_013726 Other Connector Cat# ED85100- ND; Digi- key Corporation For EEG Other Commutator Cat# 8408; Pinnacle For EEG Continued Continued on next page Chartampila et al. eLife 2023;12:RP89889.

    Article Title: Brain region and sex-dependent heterogeneity of PUFA/oxylipin profile, microglia morphology and their relationship.
    Article Snippet: Lipid dyshomeostasis and neuroinflammation are key hallmarks of neuropsychiatric and neurodegenerative disorders, including major depressive disorder and Alzheimer’s disease.. In particular, polyunsaturated fatty acids (PUFAs) and their derivatives called oxylipins gained specific interest in this context, especially considering their capacity to orchestrate neuroinflammatory responses via direct modulation of microglia.. The hippocampus and hypothalamus are crucial brain regions for regulating mood and cognition that are implicated in a variety of neuropsychiatric and neurodegenerative disorders and there is ample evidence for the sex-bias in risks for the development as well as sex-bias in the presentation of such psychiatric diseases, including the neuroinflammatory response.

    Article Title: CD45RO-Positive Memory T-Cell Density in the Tumoral Core and Invasive Margin Predict Long-Term Survival in Esophageal Squamous Cell Carcinoma.
    Article Snippet: .. For staining for biotinylated secondary antibodies, Avidin-Biotin Complex Staining kits were used (Vectastain ABC Kit, PK6100, Vector) at room temperature for 20 min. .. Slides were again washed with PBS, and the staining was visualized by incubation with DAB (Wako) for approximately 2.0 min.

    Electron Microscopy:

    Article Title: Choline supplementation in early life improves and low levels of choline can impair outcomes in a mouse model of Alzheimer’s disease
    Article Snippet: .. resource Designation Source or reference Identifiers Additional information Chemical compound, drug Paraformaldehyde (PFA) Cat# 19210; Electron Microscopy Sciences 4% Chemical compound, drug Normal goat serum Cat# S- 1000; Vector Laboratories 5% Chemical compound, drug Normal horse serum Cat# S- 2000; Vector Laboratories 5% Chemical compound, drug TRIS hydrochloride Cat# T3253; Sigma- Aldrich 97 g/8 L Chemical compound, drug Tris base (TRIZMA base) Cat# T1503; Sigma- Aldrich 22 g/8 L Chemical compound, drug H2O2; hydrogen peroxide Cat# 95321; Sigma- Aldrich 1% w/v Chemical compound, drug Avidin- biotin complex (ABC) Cat# PK6100; Vector Laboratories 1:1000 Chemical compound, drug 3,3- Diamino- benzidine (DAB) Cat# D5905; Sigma- Aldrich 0.5 mg/mL Chemical compound, drug Ammonium chloride Cat# A514; Sigma- Aldrich 40 ug/mL Chemical compound, drug D(+)- glucose Cat# G5767; Sigma- Aldrich 25 mg/mL Chemical compound, drug Glucose oxidase Cat# G2133; Sigma- Aldrich 3 g/mL Chemical compound, drug Gelatin Cat# G9391; Sigma- Aldrich 1% Chemical compound, drug Xylene Cat# 534056; Sigma- Aldrich Undiluted Chemical compound, drug Permount Cat# 17986- 01; Electron Microscopy Sciences Undiluted Chemical compound, drug Glycerol #G7893; Sigma- Aldrich 30% Chemical compound, drug Ethylene glycol Cat# 324558; Sigma- Aldrich 30% Chemical compound, drug Sterile 0.9% sodium chloride solution; saline NDC# 50989- 885- 17; Vedco, Inc Chemical compound, drug Dental cement Cat# 4734FIB, Lang Dental Mfg. .. Co Chemical compound, drug Lactated Ringer’s solution NDC# 099355000476; Aspen Veterinary Resources Ltd 50 mL/kg Chemical compound, drug Isoflurane NDC# 07- 893- 1389; Patterson Veterinary Chemical compound, drug Urethane Cat# U2500; Sigma- Aldrich 2.5 g/kg Chemical compound, drug Buprenorphine Buprenex; NDC#12496- 075705; Reckitt Benckiser 0.2 mg/kg Strain, strain background (Mus musculus) Tg2576 mice, C57BL6/SJL background Jackson Labs Stock# 100012 Software, algorithm ImagePro Plus V7.0 Media Cybernetics Software, algorithm Sirenia acquisition Pinnacle Technology RRID:SCR_016183 Software, algorithm Sirenia Seizure Pinnacle Technology RRID:SCR_016184 Software, algorithm Neuroscore Data Science International Software, algorithm Prism GraphPad RRID:SCR_002798 Software, algorithm Photoshop Adobe RRID:SCR_014199 Software, algorithm ImageJ NIH RRID:SCR_003070 Version 1.44 Software, algorithm G*Power G*Power RRID:SCR_013726 Other Connector Cat# ED85100- ND; Digi- key Corporation For EEG Other Commutator Cat# 8408; Pinnacle For EEG Continued Continued on next page Chartampila et al. eLife 2023;12:RP89889.

    Sterility:

    Article Title: Choline supplementation in early life improves and low levels of choline can impair outcomes in a mouse model of Alzheimer’s disease
    Article Snippet: .. resource Designation Source or reference Identifiers Additional information Chemical compound, drug Paraformaldehyde (PFA) Cat# 19210; Electron Microscopy Sciences 4% Chemical compound, drug Normal goat serum Cat# S- 1000; Vector Laboratories 5% Chemical compound, drug Normal horse serum Cat# S- 2000; Vector Laboratories 5% Chemical compound, drug TRIS hydrochloride Cat# T3253; Sigma- Aldrich 97 g/8 L Chemical compound, drug Tris base (TRIZMA base) Cat# T1503; Sigma- Aldrich 22 g/8 L Chemical compound, drug H2O2; hydrogen peroxide Cat# 95321; Sigma- Aldrich 1% w/v Chemical compound, drug Avidin- biotin complex (ABC) Cat# PK6100; Vector Laboratories 1:1000 Chemical compound, drug 3,3- Diamino- benzidine (DAB) Cat# D5905; Sigma- Aldrich 0.5 mg/mL Chemical compound, drug Ammonium chloride Cat# A514; Sigma- Aldrich 40 ug/mL Chemical compound, drug D(+)- glucose Cat# G5767; Sigma- Aldrich 25 mg/mL Chemical compound, drug Glucose oxidase Cat# G2133; Sigma- Aldrich 3 g/mL Chemical compound, drug Gelatin Cat# G9391; Sigma- Aldrich 1% Chemical compound, drug Xylene Cat# 534056; Sigma- Aldrich Undiluted Chemical compound, drug Permount Cat# 17986- 01; Electron Microscopy Sciences Undiluted Chemical compound, drug Glycerol #G7893; Sigma- Aldrich 30% Chemical compound, drug Ethylene glycol Cat# 324558; Sigma- Aldrich 30% Chemical compound, drug Sterile 0.9% sodium chloride solution; saline NDC# 50989- 885- 17; Vedco, Inc Chemical compound, drug Dental cement Cat# 4734FIB, Lang Dental Mfg. .. Co Chemical compound, drug Lactated Ringer’s solution NDC# 099355000476; Aspen Veterinary Resources Ltd 50 mL/kg Chemical compound, drug Isoflurane NDC# 07- 893- 1389; Patterson Veterinary Chemical compound, drug Urethane Cat# U2500; Sigma- Aldrich 2.5 g/kg Chemical compound, drug Buprenorphine Buprenex; NDC#12496- 075705; Reckitt Benckiser 0.2 mg/kg Strain, strain background (Mus musculus) Tg2576 mice, C57BL6/SJL background Jackson Labs Stock# 100012 Software, algorithm ImagePro Plus V7.0 Media Cybernetics Software, algorithm Sirenia acquisition Pinnacle Technology RRID:SCR_016183 Software, algorithm Sirenia Seizure Pinnacle Technology RRID:SCR_016184 Software, algorithm Neuroscore Data Science International Software, algorithm Prism GraphPad RRID:SCR_002798 Software, algorithm Photoshop Adobe RRID:SCR_014199 Software, algorithm ImageJ NIH RRID:SCR_003070 Version 1.44 Software, algorithm G*Power G*Power RRID:SCR_013726 Other Connector Cat# ED85100- ND; Digi- key Corporation For EEG Other Commutator Cat# 8408; Pinnacle For EEG Continued Continued on next page Chartampila et al. eLife 2023;12:RP89889.

    Saline:

    Article Title: Choline supplementation in early life improves and low levels of choline can impair outcomes in a mouse model of Alzheimer’s disease
    Article Snippet: .. resource Designation Source or reference Identifiers Additional information Chemical compound, drug Paraformaldehyde (PFA) Cat# 19210; Electron Microscopy Sciences 4% Chemical compound, drug Normal goat serum Cat# S- 1000; Vector Laboratories 5% Chemical compound, drug Normal horse serum Cat# S- 2000; Vector Laboratories 5% Chemical compound, drug TRIS hydrochloride Cat# T3253; Sigma- Aldrich 97 g/8 L Chemical compound, drug Tris base (TRIZMA base) Cat# T1503; Sigma- Aldrich 22 g/8 L Chemical compound, drug H2O2; hydrogen peroxide Cat# 95321; Sigma- Aldrich 1% w/v Chemical compound, drug Avidin- biotin complex (ABC) Cat# PK6100; Vector Laboratories 1:1000 Chemical compound, drug 3,3- Diamino- benzidine (DAB) Cat# D5905; Sigma- Aldrich 0.5 mg/mL Chemical compound, drug Ammonium chloride Cat# A514; Sigma- Aldrich 40 ug/mL Chemical compound, drug D(+)- glucose Cat# G5767; Sigma- Aldrich 25 mg/mL Chemical compound, drug Glucose oxidase Cat# G2133; Sigma- Aldrich 3 g/mL Chemical compound, drug Gelatin Cat# G9391; Sigma- Aldrich 1% Chemical compound, drug Xylene Cat# 534056; Sigma- Aldrich Undiluted Chemical compound, drug Permount Cat# 17986- 01; Electron Microscopy Sciences Undiluted Chemical compound, drug Glycerol #G7893; Sigma- Aldrich 30% Chemical compound, drug Ethylene glycol Cat# 324558; Sigma- Aldrich 30% Chemical compound, drug Sterile 0.9% sodium chloride solution; saline NDC# 50989- 885- 17; Vedco, Inc Chemical compound, drug Dental cement Cat# 4734FIB, Lang Dental Mfg. .. Co Chemical compound, drug Lactated Ringer’s solution NDC# 099355000476; Aspen Veterinary Resources Ltd 50 mL/kg Chemical compound, drug Isoflurane NDC# 07- 893- 1389; Patterson Veterinary Chemical compound, drug Urethane Cat# U2500; Sigma- Aldrich 2.5 g/kg Chemical compound, drug Buprenorphine Buprenex; NDC#12496- 075705; Reckitt Benckiser 0.2 mg/kg Strain, strain background (Mus musculus) Tg2576 mice, C57BL6/SJL background Jackson Labs Stock# 100012 Software, algorithm ImagePro Plus V7.0 Media Cybernetics Software, algorithm Sirenia acquisition Pinnacle Technology RRID:SCR_016183 Software, algorithm Sirenia Seizure Pinnacle Technology RRID:SCR_016184 Software, algorithm Neuroscore Data Science International Software, algorithm Prism GraphPad RRID:SCR_002798 Software, algorithm Photoshop Adobe RRID:SCR_014199 Software, algorithm ImageJ NIH RRID:SCR_003070 Version 1.44 Software, algorithm G*Power G*Power RRID:SCR_013726 Other Connector Cat# ED85100- ND; Digi- key Corporation For EEG Other Commutator Cat# 8408; Pinnacle For EEG Continued Continued on next page Chartampila et al. eLife 2023;12:RP89889.

    other:

    Article Title: GABA co-released from striatal dopamine axons dampens phasic dopamine release through autoregulatory GABA A receptors
    Article Snippet: Vectastain Elite ABC-HRP Kit , Vector Labs , Cat No: PK6100.

    cDNA Synthesis:

    Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Digitonin, high purity Millipore Cat# 300410 DMEM Gibco Cat# 10569-010 DMEM/F12 Gibco Cat# 11330-032 DMSO Sigma-Aldrich Cat# D2650-100ML Donkey serum EMD Millipore Corp. Cat# S30-100ML eBioscience Fixable Viability Dye eFluor 780 Invitrogen Cat# 65-0865-18 EDTA (perfusion) Promega Cat# V4231 Ethanol Sigma-Aldrich Cat# E7023-500ML Ethylene glycol Sigma-Aldrich Cat# 102466-4L FBS One-Shot Heat Inactivated Gibco Cat# A38400-01 GlutaMAX Gibco Cat# 35050-061 GM-CSF BioLegend Cat# 576304 HBSS Gibco Cat# 14175-079 Heparin Hepalink USA Inc. Cat# NDC 81952-112-05 Hoechst 33342 Invitrogen Cat# H3570 Hydrogen peroxide Fisher Cat# H325-500 ITS Supplement R&D Systems Cat# AR013 LipidTox Green Neutral Lipid Stain Invitrogen Cat# H34475 LPS O111:B4 Sigma-Aldrich Cat# L5293-2ML M-CSF BioLegend Cat# 576404 Milk powder Research Products International Cat# M17200–1000.0 M-PER Thermo Scientific Cat# 78501 Na 2 EDTA Sigma-Aldrich Cat# O3690-100ML Nickel chloride Sigma-Aldrich Cat# N-5756 Oleic acid-BSA Cayman Cat# 29557 Penicillin-streptomycin Gibco Cat# 15140-122 PF-06424439 DGAT2 inhibitor Sigma-Aldrich Cat# PZ0233-5MG PFA (cell culture) Electron Microscopy Sciences Cat# 15710 PFA (perfusion) Electron Microscopy Sciences Cat# 19202 Pierce BCA Protein Assay Thermo Scientific Cat# 23225 Poly-L-lysine Sigma-Aldrich Cat# P4707-50ML Prolong Gold Invitrogen Cat# P36934 Prolong Live Antifade Reagent Invitrogen Cat# P36975 Sodium pyruvate Corning Cat# 25-000-CI Sucrose Fisher Cat# S5-3 T863 DGAT1 inhibitor Sigma-Aldrich Cat# SML-0539-5MG Tamoxifen Sigma-Aldrich Cat# T5648 1M Tris-HCl, pH 8.0 Corning Cat# 46-031-CM Triton X-100 Sigma-Aldrich Cat# X-100-500ML True Black Biotium Cat# 23007 Trypsin-EDTA 0.25% Gibco Cat# 25200-056 Critical commercial assays 10X Genomics Chromium GEM-X Single Cell 3 ′ V4 kit 10X Genomics – Adult Brain Dissociation Kit Miltenyi Cat# 130-107-677 LS columns Miltenyi Cat# 130-042-401 Pierce BCA Protein Assay Kit Thermo Scientific Cat# 23225 Qubit RNA BR Assay Invitrogen Cat# Q10210 RNeasy Micro Plus Kit Qiagen Cat# 74034 (Continued on next page) Cell Reports 45, 116841, January 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Simoa NF-Light Advantage PLUS Reagent Kit Quanterix Cat# 104364 Superscript IV VILO cDNA synthesis kit Invitrogen Cat# 11766050 Taqman Fast Advanced Master Mix Applied Biosystems Cat# 4444556 VECTASTAIN Elite ABC-HRP Kit Peroxidase (Standard) Vector Laboratories Cat# PK6100 Deposited data Mouse snRNA-seq data for the PU.1-sorted hippocampal samples This paper GEO: GSE286044 Mouse snRNA-seq data for the bulk cortical samples This paper EMBL-EBI: E-MTAB-16169 Lipidomic data for the bulk posterior cortical samples This paper Metabolomics Workbench: PR002778; https://doi.org/10.21228/M8K84F Experimental models: Organisms/strains Mouse: Cx3cr1-CreERT2 (Littman) Jackson Laboratory Cat# 021160; RRID:IMSR_JAX:021160 Mouse: DGAT1 flox/flox Jackson Laboratory Cat# 017322; RRID:IMSR_JAX:017322 Mouse: DGAT2 flox/flox Jackson Laboratory Cat# 033518; RRID:IMSR_JAX:033518 Mouse: PS19 (P301S Tau) line (B6; C3-Tg (Prnp MAPT*P301S) PS19Vle/J) Jackson Laboratory Cat# 008169; RRID: IMSR_JAX:008169 Oligonucleotides DGAT1 Taqman Assay ThermoFisher Cat# Mm00515643_m1, FAM-MGB, #4331182 DGAT2 Taqman Assay ThermoFisher Cat# Mm00499536_m1, FAM-MGB, #4331182 GAPDH Taqman Assay ThermoFisher Cat# MM99999915_g1, VIC, #4448489 Software and algorithms Cell Ranger v8.0.1. .. 2.0 10X Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest FreezeFrame Actimetrics https://actimetrics.com/products/freezeframe/ Gen5 3.14 Agilent https://www.agilent.com/en/product/ cell-analysis/cell-imaging-microscopy/ cell-imaging-microscopy-software/biotekgen5-software-for-imagingmicroscopy-1623226 ImageJ 1.54m NIH https://imagej.net/ij/ Imaris Oxford Instruments https://imaris.oxinst.com/packages LASX Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/ NDP.view 2.9.29 Hamamatsu https://www.hamamatsu.com/jp/en/ product/life-science-and-medicalsystems/digital-slide-scanner/ U12388-01.html Prism 10 GraphPad https://www.graphpad.com/features QuantStudio v1.5.3 Applied Biosystems https://www.thermofisher.com/us/ en/home/technical-resources/ software-downloads/quantstudio3-5-real-time-pcr-systems.html R R https://www.r-project.org/ Seurat Rahul Satija Lab https://satijalab.org/seurat/ Zeiss Zen 3.0 Zeiss https://www.zeiss.com/microscopy/ us/products/software/zeiss-zen.html Zotero 6.0.36 Digital Scholar https://www.zotero.org/download/ Other #1.5 glass coverslips Tanner Scientific Cat# TNR 24501.5 70 μm cell strainers PRIMA Cat# 70ICS (Continued on next page) 20 Cell Reports 45, 116841, January 27, 2026

    TaqMan Assay:

    Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Digitonin, high purity Millipore Cat# 300410 DMEM Gibco Cat# 10569-010 DMEM/F12 Gibco Cat# 11330-032 DMSO Sigma-Aldrich Cat# D2650-100ML Donkey serum EMD Millipore Corp. Cat# S30-100ML eBioscience Fixable Viability Dye eFluor 780 Invitrogen Cat# 65-0865-18 EDTA (perfusion) Promega Cat# V4231 Ethanol Sigma-Aldrich Cat# E7023-500ML Ethylene glycol Sigma-Aldrich Cat# 102466-4L FBS One-Shot Heat Inactivated Gibco Cat# A38400-01 GlutaMAX Gibco Cat# 35050-061 GM-CSF BioLegend Cat# 576304 HBSS Gibco Cat# 14175-079 Heparin Hepalink USA Inc. Cat# NDC 81952-112-05 Hoechst 33342 Invitrogen Cat# H3570 Hydrogen peroxide Fisher Cat# H325-500 ITS Supplement R&D Systems Cat# AR013 LipidTox Green Neutral Lipid Stain Invitrogen Cat# H34475 LPS O111:B4 Sigma-Aldrich Cat# L5293-2ML M-CSF BioLegend Cat# 576404 Milk powder Research Products International Cat# M17200–1000.0 M-PER Thermo Scientific Cat# 78501 Na 2 EDTA Sigma-Aldrich Cat# O3690-100ML Nickel chloride Sigma-Aldrich Cat# N-5756 Oleic acid-BSA Cayman Cat# 29557 Penicillin-streptomycin Gibco Cat# 15140-122 PF-06424439 DGAT2 inhibitor Sigma-Aldrich Cat# PZ0233-5MG PFA (cell culture) Electron Microscopy Sciences Cat# 15710 PFA (perfusion) Electron Microscopy Sciences Cat# 19202 Pierce BCA Protein Assay Thermo Scientific Cat# 23225 Poly-L-lysine Sigma-Aldrich Cat# P4707-50ML Prolong Gold Invitrogen Cat# P36934 Prolong Live Antifade Reagent Invitrogen Cat# P36975 Sodium pyruvate Corning Cat# 25-000-CI Sucrose Fisher Cat# S5-3 T863 DGAT1 inhibitor Sigma-Aldrich Cat# SML-0539-5MG Tamoxifen Sigma-Aldrich Cat# T5648 1M Tris-HCl, pH 8.0 Corning Cat# 46-031-CM Triton X-100 Sigma-Aldrich Cat# X-100-500ML True Black Biotium Cat# 23007 Trypsin-EDTA 0.25% Gibco Cat# 25200-056 Critical commercial assays 10X Genomics Chromium GEM-X Single Cell 3 ′ V4 kit 10X Genomics – Adult Brain Dissociation Kit Miltenyi Cat# 130-107-677 LS columns Miltenyi Cat# 130-042-401 Pierce BCA Protein Assay Kit Thermo Scientific Cat# 23225 Qubit RNA BR Assay Invitrogen Cat# Q10210 RNeasy Micro Plus Kit Qiagen Cat# 74034 (Continued on next page) Cell Reports 45, 116841, January 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Simoa NF-Light Advantage PLUS Reagent Kit Quanterix Cat# 104364 Superscript IV VILO cDNA synthesis kit Invitrogen Cat# 11766050 Taqman Fast Advanced Master Mix Applied Biosystems Cat# 4444556 VECTASTAIN Elite ABC-HRP Kit Peroxidase (Standard) Vector Laboratories Cat# PK6100 Deposited data Mouse snRNA-seq data for the PU.1-sorted hippocampal samples This paper GEO: GSE286044 Mouse snRNA-seq data for the bulk cortical samples This paper EMBL-EBI: E-MTAB-16169 Lipidomic data for the bulk posterior cortical samples This paper Metabolomics Workbench: PR002778; https://doi.org/10.21228/M8K84F Experimental models: Organisms/strains Mouse: Cx3cr1-CreERT2 (Littman) Jackson Laboratory Cat# 021160; RRID:IMSR_JAX:021160 Mouse: DGAT1 flox/flox Jackson Laboratory Cat# 017322; RRID:IMSR_JAX:017322 Mouse: DGAT2 flox/flox Jackson Laboratory Cat# 033518; RRID:IMSR_JAX:033518 Mouse: PS19 (P301S Tau) line (B6; C3-Tg (Prnp MAPT*P301S) PS19Vle/J) Jackson Laboratory Cat# 008169; RRID: IMSR_JAX:008169 Oligonucleotides DGAT1 Taqman Assay ThermoFisher Cat# Mm00515643_m1, FAM-MGB, #4331182 DGAT2 Taqman Assay ThermoFisher Cat# Mm00499536_m1, FAM-MGB, #4331182 GAPDH Taqman Assay ThermoFisher Cat# MM99999915_g1, VIC, #4448489 Software and algorithms Cell Ranger v8.0.1. .. 2.0 10X Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest FreezeFrame Actimetrics https://actimetrics.com/products/freezeframe/ Gen5 3.14 Agilent https://www.agilent.com/en/product/ cell-analysis/cell-imaging-microscopy/ cell-imaging-microscopy-software/biotekgen5-software-for-imagingmicroscopy-1623226 ImageJ 1.54m NIH https://imagej.net/ij/ Imaris Oxford Instruments https://imaris.oxinst.com/packages LASX Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/ NDP.view 2.9.29 Hamamatsu https://www.hamamatsu.com/jp/en/ product/life-science-and-medicalsystems/digital-slide-scanner/ U12388-01.html Prism 10 GraphPad https://www.graphpad.com/features QuantStudio v1.5.3 Applied Biosystems https://www.thermofisher.com/us/ en/home/technical-resources/ software-downloads/quantstudio3-5-real-time-pcr-systems.html R R https://www.r-project.org/ Seurat Rahul Satija Lab https://satijalab.org/seurat/ Zeiss Zen 3.0 Zeiss https://www.zeiss.com/microscopy/ us/products/software/zeiss-zen.html Zotero 6.0.36 Digital Scholar https://www.zotero.org/download/ Other #1.5 glass coverslips Tanner Scientific Cat# TNR 24501.5 70 μm cell strainers PRIMA Cat# 70ICS (Continued on next page) 20 Cell Reports 45, 116841, January 27, 2026

    Software:

    Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Digitonin, high purity Millipore Cat# 300410 DMEM Gibco Cat# 10569-010 DMEM/F12 Gibco Cat# 11330-032 DMSO Sigma-Aldrich Cat# D2650-100ML Donkey serum EMD Millipore Corp. Cat# S30-100ML eBioscience Fixable Viability Dye eFluor 780 Invitrogen Cat# 65-0865-18 EDTA (perfusion) Promega Cat# V4231 Ethanol Sigma-Aldrich Cat# E7023-500ML Ethylene glycol Sigma-Aldrich Cat# 102466-4L FBS One-Shot Heat Inactivated Gibco Cat# A38400-01 GlutaMAX Gibco Cat# 35050-061 GM-CSF BioLegend Cat# 576304 HBSS Gibco Cat# 14175-079 Heparin Hepalink USA Inc. Cat# NDC 81952-112-05 Hoechst 33342 Invitrogen Cat# H3570 Hydrogen peroxide Fisher Cat# H325-500 ITS Supplement R&D Systems Cat# AR013 LipidTox Green Neutral Lipid Stain Invitrogen Cat# H34475 LPS O111:B4 Sigma-Aldrich Cat# L5293-2ML M-CSF BioLegend Cat# 576404 Milk powder Research Products International Cat# M17200–1000.0 M-PER Thermo Scientific Cat# 78501 Na 2 EDTA Sigma-Aldrich Cat# O3690-100ML Nickel chloride Sigma-Aldrich Cat# N-5756 Oleic acid-BSA Cayman Cat# 29557 Penicillin-streptomycin Gibco Cat# 15140-122 PF-06424439 DGAT2 inhibitor Sigma-Aldrich Cat# PZ0233-5MG PFA (cell culture) Electron Microscopy Sciences Cat# 15710 PFA (perfusion) Electron Microscopy Sciences Cat# 19202 Pierce BCA Protein Assay Thermo Scientific Cat# 23225 Poly-L-lysine Sigma-Aldrich Cat# P4707-50ML Prolong Gold Invitrogen Cat# P36934 Prolong Live Antifade Reagent Invitrogen Cat# P36975 Sodium pyruvate Corning Cat# 25-000-CI Sucrose Fisher Cat# S5-3 T863 DGAT1 inhibitor Sigma-Aldrich Cat# SML-0539-5MG Tamoxifen Sigma-Aldrich Cat# T5648 1M Tris-HCl, pH 8.0 Corning Cat# 46-031-CM Triton X-100 Sigma-Aldrich Cat# X-100-500ML True Black Biotium Cat# 23007 Trypsin-EDTA 0.25% Gibco Cat# 25200-056 Critical commercial assays 10X Genomics Chromium GEM-X Single Cell 3 ′ V4 kit 10X Genomics – Adult Brain Dissociation Kit Miltenyi Cat# 130-107-677 LS columns Miltenyi Cat# 130-042-401 Pierce BCA Protein Assay Kit Thermo Scientific Cat# 23225 Qubit RNA BR Assay Invitrogen Cat# Q10210 RNeasy Micro Plus Kit Qiagen Cat# 74034 (Continued on next page) Cell Reports 45, 116841, January 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Simoa NF-Light Advantage PLUS Reagent Kit Quanterix Cat# 104364 Superscript IV VILO cDNA synthesis kit Invitrogen Cat# 11766050 Taqman Fast Advanced Master Mix Applied Biosystems Cat# 4444556 VECTASTAIN Elite ABC-HRP Kit Peroxidase (Standard) Vector Laboratories Cat# PK6100 Deposited data Mouse snRNA-seq data for the PU.1-sorted hippocampal samples This paper GEO: GSE286044 Mouse snRNA-seq data for the bulk cortical samples This paper EMBL-EBI: E-MTAB-16169 Lipidomic data for the bulk posterior cortical samples This paper Metabolomics Workbench: PR002778; https://doi.org/10.21228/M8K84F Experimental models: Organisms/strains Mouse: Cx3cr1-CreERT2 (Littman) Jackson Laboratory Cat# 021160; RRID:IMSR_JAX:021160 Mouse: DGAT1 flox/flox Jackson Laboratory Cat# 017322; RRID:IMSR_JAX:017322 Mouse: DGAT2 flox/flox Jackson Laboratory Cat# 033518; RRID:IMSR_JAX:033518 Mouse: PS19 (P301S Tau) line (B6; C3-Tg (Prnp MAPT*P301S) PS19Vle/J) Jackson Laboratory Cat# 008169; RRID: IMSR_JAX:008169 Oligonucleotides DGAT1 Taqman Assay ThermoFisher Cat# Mm00515643_m1, FAM-MGB, #4331182 DGAT2 Taqman Assay ThermoFisher Cat# Mm00499536_m1, FAM-MGB, #4331182 GAPDH Taqman Assay ThermoFisher Cat# MM99999915_g1, VIC, #4448489 Software and algorithms Cell Ranger v8.0.1. .. 2.0 10X Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest FreezeFrame Actimetrics https://actimetrics.com/products/freezeframe/ Gen5 3.14 Agilent https://www.agilent.com/en/product/ cell-analysis/cell-imaging-microscopy/ cell-imaging-microscopy-software/biotekgen5-software-for-imagingmicroscopy-1623226 ImageJ 1.54m NIH https://imagej.net/ij/ Imaris Oxford Instruments https://imaris.oxinst.com/packages LASX Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/ NDP.view 2.9.29 Hamamatsu https://www.hamamatsu.com/jp/en/ product/life-science-and-medicalsystems/digital-slide-scanner/ U12388-01.html Prism 10 GraphPad https://www.graphpad.com/features QuantStudio v1.5.3 Applied Biosystems https://www.thermofisher.com/us/ en/home/technical-resources/ software-downloads/quantstudio3-5-real-time-pcr-systems.html R R https://www.r-project.org/ Seurat Rahul Satija Lab https://satijalab.org/seurat/ Zeiss Zen 3.0 Zeiss https://www.zeiss.com/microscopy/ us/products/software/zeiss-zen.html Zotero 6.0.36 Digital Scholar https://www.zotero.org/download/ Other #1.5 glass coverslips Tanner Scientific Cat# TNR 24501.5 70 μm cell strainers PRIMA Cat# 70ICS (Continued on next page) 20 Cell Reports 45, 116841, January 27, 2026

    Article Title: Early and selective localization of tau filaments to glutamatergic subcellular domains within the human anterodorsal thalamus
    Article Snippet: .. Chemicals Normal goat serum Vector Laboratories Cat# S-1000 Normal horse serum Vector Laboratories Cat# S-2000 Cold Water Fish Skin Gelatin Aurion Cat# 900.033 Enhancement conditioning solution (ECS) Aurion Cat# 500.055 Silver enhancement solution SELM Aurion Cat# 500.022 Diaminobenzidine (DAB) Sigma-Aldrich Cat# D5637-1G Chromium(III) potassium sulfate dodecahydrate Sigma-Aldrich Cat# 243361 Gelatin from bovine skin Sigma-Aldrich Cat# G9391 Acetonitrile VWR Chemicals Cat# 83657.32 DPX Mountant Merck Cat# 6522 Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21202 Donkey anti-rabbit Cy3 Jackson Immuno Research Cat# 711-165-152 Biotinylated Goat anti-mouse Vector Laboratories Cat# BA-9200-1.5 Goat-anti-Mouse IgG (H&L) Ultra Small Aurion Cat# 800.022 Critical commercial assays Vectastain ABC Elite kit Vector Laboratories Cat# PK6100; RRID: AB_2336819 Vectashield Antifade Mounting Medium Vector Laboratories Cat# H-1000; RRID: AB_2336789 TSA Biotin Reagent Pack Akoya Biosciences Cat# SAT700001EA Software and algorithms Fiji ImageJ https://imagej.net/software/fiji Zen 2008, Zen Black, Zen Blue, Zeiss www.zeiss.co.uk Python https://www.python.org/ CaseViewer 3DHISTECH https://www.3dhistech.com/solutions/caseviewer QuPath Bankhead et al. https://qupath.github.io/ TrakEM2 Cardona et al. https://www.ini.uzh.ch/~acardona/trakem2.html Table S2. ..

    Incubation:

    Article Title: Brain region and sex-dependent heterogeneity of PUFA/oxylipin profile, microglia morphology and their relationship.
    Article Snippet: Lipid dyshomeostasis and neuroinflammation are key hallmarks of neuropsychiatric and neurodegenerative disorders, including major depressive disorder and Alzheimer’s disease.. In particular, polyunsaturated fatty acids (PUFAs) and their derivatives called oxylipins gained specific interest in this context, especially considering their capacity to orchestrate neuroinflammatory responses via direct modulation of microglia.. The hippocampus and hypothalamus are crucial brain regions for regulating mood and cognition that are implicated in a variety of neuropsychiatric and neurodegenerative disorders and there is ample evidence for the sex-bias in risks for the development as well as sex-bias in the presentation of such psychiatric diseases, including the neuroinflammatory response.

    Staining:

    Article Title: CD45RO-Positive Memory T-Cell Density in the Tumoral Core and Invasive Margin Predict Long-Term Survival in Esophageal Squamous Cell Carcinoma.
    Article Snippet: .. For staining for biotinylated secondary antibodies, Avidin-Biotin Complex Staining kits were used (Vectastain ABC Kit, PK6100, Vector) at room temperature for 20 min. .. Slides were again washed with PBS, and the staining was visualized by incubation with DAB (Wako) for approximately 2.0 min.



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